This program provides a comprehensive guide to Hematoxylin and Eosin (H&E) staining, the gold standard in diagnostic pathology. It covers the chemical properties of dyes, the role of mordants, and the essential steps of tissue processing required for high-quality slides. Students will examine specific staining protocols, learn to differentiate between progressive and regressive methods, and identify common artifacts that impact diagnostic clarity. The course is designed for histotechnology professionals who aim to maintain rigorous academic and clinical standards in the laboratory.